A rapid method for monitoring DNA labelling reactions with haptens

A rapid method for monitoring DNA labelling reactions with haptens

A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary...

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Veröffentlicht in:Journal of immunological methods 1990-11, Vol.134 (1), p.81-86
Hauptverfasser: Corti, Angelo, Tripputi, Pasquale, Cassani, Giovanni
Format: Artikel
Sprache:eng
Schlagworte:
Alkaline Phosphatase - chemistry
Animals
Biological and medical sciences
Digoxigenin
DNA - analysis
DNA - chemistry
Enzyme immunoassay
Filtration
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Haptens - analysis
Haptens - chemistry
Haptens - immunology
Hybridisation assay
Immunoassay - methods
Mice
Molecular immunology
Nonradioactive labelling
Nucleic acid
Plasmids
Sulfones
Techniques
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Zusammenfassung:A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(90)90114-B