Matrix Metalloproteinase-2 and −9 Differentially Regulate Smooth Muscle Cell Migration and Cell-Mediated Collagen Organization

OBJECTIVES—Smooth muscle cells (SMCs) produce both matrix metalloproteinase (MMP)-2 and MMP-9, enzymes with similar in vitro matrix degrading abilities. We compared the specific contributions of these enzymes to SMC-matrix interactions in vitro and in vivo. METHODS AND RESULTS—Using genetic models o...

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Veröffentlicht in:	Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2004-01, Vol.24 (1), p.54-60
Hauptverfasser:	Johnson, Chad, Galis, Zorina S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Cell Adhesion Cell Movement - drug effects Cells, Cultured Collagen - metabolism Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Extracellular Matrix - metabolism Gels Hyaluronan Receptors - physiology Hyperplasia Matrix Metalloproteinase 2 - deficiency Matrix Metalloproteinase 2 - genetics Matrix Metalloproteinase 2 - physiology Matrix Metalloproteinase 9 - deficiency Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - physiology Medical sciences Mice Mice, Inbred C57BL Mice, Knockout Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - enzymology Platelet-Derived Growth Factor - pharmacology Protease Inhibitors - pharmacology Proto-Oncogene Proteins c-sis Tunica Intima - pathology
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Zusammenfassung:	OBJECTIVES—Smooth muscle cells (SMCs) produce both matrix metalloproteinase (MMP)-2 and MMP-9, enzymes with similar in vitro matrix degrading abilities. We compared the specific contributions of these enzymes to SMC-matrix interactions in vitro and in vivo. METHODS AND RESULTS—Using genetic models of deficiency, we investigated MMP-2 and MMP-9 roles in SMC migration in vivo in the formation of intimal hyperplasia and in vitro. In addition, we investigated potential effects of MMP-2 and MMP-9 genetic deficiency on compaction and assembly of collagen by SMCs. CONCLUSIONS—MMP-2 and MMP-9 genetic deficiency decreased by 81% and 65%, respectively (P
ISSN:	1079-5642 1524-4636
DOI:	10.1161/01.ATV.0000100402.69997.C3