Protein differences between normal and oligospermic human sperm demonstrated by two-dimensional gel electrophoresis

Protein expression by sperm obtained from men with normal semen analysis and men with oligospermia were evaluated by two-dimensional gel electrophoresis. Proteins were solubilized in a 9.5 M urea/2% Nonidet-P40 (LKB, Bromma, Sweden) lysis buffer and underwent second dimension separation on 10 to 16%...

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Veröffentlicht in:	Fertility and sterility 1990-11, Vol.54 (5), p.902-905
Hauptverfasser:	Morgentaler, Abraham, Schopperle, William M., Crocker, Roderick H., DeWolf, William C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biomarkers Birth control Electrophoresis, Gel, Two-Dimensional Gynecology. Andrology. Obstetrics Humans Infertility, Male - metabolism Male Medical sciences Oligospermia - metabolism Oligospermia - physiopathology Proteins - analysis Sperm Motility - physiology Spermatozoa - chemistry Spermatozoa - metabolism Spermatozoa - physiology Sterility. Assisted procreation
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Zusammenfassung:	Protein expression by sperm obtained from men with normal semen analysis and men with oligospermia were evaluated by two-dimensional gel electrophoresis. Proteins were solubilized in a 9.5 M urea/2% Nonidet-P40 (LKB, Bromma, Sweden) lysis buffer and underwent second dimension separation on 10 to 16% polyacrylamide gradient gels. A set of 36 invariant proteins was identified in all normospermic samples, whereas 8 of 10 evaluable oligospermic samples lacked 1 or more of the invariant proteins. Proteins absent in oligospermic samples may be critical to normal sperm function and may serve as markers for infertility.
ISSN:	0015-0282 1556-5653
DOI:	10.1016/S0015-0282(16)53953-3