Adenosine system and cell calcium translocation: Interference of calcium channel blockers
Adenosine is able to inhibit in vitro neutrophil functions induced by formyl-methionyl-leucyl-phenylalanine (FMLP) and A23187, but not phorbol 12-myristate 13-acetate (PMA). The inhibiting activity on A23187 is reversed by increasing extracellular Ca ++ concentration. The calcium entry blocker fluna...
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Veröffentlicht in: | Experimental gerontology 1990, Vol.25 (3), p.383-391 |
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Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Adenosine is able to inhibit in vitro neutrophil functions induced by formyl-methionyl-leucyl-phenylalanine (FMLP) and A23187, but not phorbol 12-myristate 13-acetate (PMA). The inhibiting activity on A23187 is reversed by increasing extracellular Ca
++ concentration. The calcium entry blocker flunarizine shows an activity very similar to that of adenosine. Both adenosine and flunarizine prevent Ca
++ influx into activated neutrophils as detected by the fluorescent Ca
++ chelator Quin-2. Finally, flunarizine binds to the neutrophil membrane and adenosine competitively inhibits flunarizine binding as assessed by
1H-Nuclear Magnetic Resonance (
1H-NMR) technique, thus indicating that the two agents share a common binding site on the cell membrane. |
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ISSN: | 0531-5565 1873-6815 |
DOI: | 10.1016/0531-5565(90)90076-E |