The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii
The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[ 35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 2...
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| Veröffentlicht in: | FEBS letters 1990-09, Vol.270 (1), p.132-134 |
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| container_title | FEBS letters |
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| creator | Korolkov, S.N. Garnovskaya, M.N. Basov, A.S. Chunaev, A.S. Dumler, I.L. |
| description | The presence of G-proteins in the eyespot fraction of
Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[
35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent
M
r 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of
Ch. reinhardtii. |
| doi_str_mv | 10.1016/0014-5793(90)81251-I |
| format | Article |
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Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[
35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent
M
r 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of
Ch. reinhardtii.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(90)81251-I</identifier><identifier>PMID: 2121520</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Antibodies ; Binding and carrier proteins ; Biological and medical sciences ; Chlamydomonas - physiology ; Chlamydomonas reinhardtii ; Fundamental and applied biological sciences. Psychology ; G-protein ; GTP Phosphohydrolases - physiology ; GTP-Binding Proteins - isolation & purification ; GTP-Binding Proteins - physiology ; GTPase ; GTPγ[ 35S] ; Guanosine 5'-O-(3-Thiotriphosphate) - metabolism ; Immunoblotting ; Light ; Molecular Weight ; Proteins ; Rhodopsin ; Sulfur Radioisotopes ; Transducin - physiology</subject><ispartof>FEBS letters, 1990-09, Vol.270 (1), p.132-134</ispartof><rights>1990</rights><rights>FEBS Letters 270 (1990) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496I-e561009bb84b8369e5f570f90bb9c808e4a3bc3b9aec8f22b5b36e89e82d743</citedby><cites>FETCH-LOGICAL-c496I-e561009bb84b8369e5f570f90bb9c808e4a3bc3b9aec8f22b5b36e89e82d743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(90)81251-I$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19788120$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2121520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Korolkov, S.N.</creatorcontrib><creatorcontrib>Garnovskaya, M.N.</creatorcontrib><creatorcontrib>Basov, A.S.</creatorcontrib><creatorcontrib>Chunaev, A.S.</creatorcontrib><creatorcontrib>Dumler, I.L.</creatorcontrib><title>The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The presence of G-proteins in the eyespot fraction of
Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[
35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent
M
r 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of
Ch. reinhardtii.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antibodies</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Chlamydomonas - physiology</subject><subject>Chlamydomonas reinhardtii</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G-protein</subject><subject>GTP Phosphohydrolases - physiology</subject><subject>GTP-Binding Proteins - isolation & purification</subject><subject>GTP-Binding Proteins - physiology</subject><subject>GTPase</subject><subject>GTPγ[ 35S]</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate) - metabolism</subject><subject>Immunoblotting</subject><subject>Light</subject><subject>Molecular Weight</subject><subject>Proteins</subject><subject>Rhodopsin</subject><subject>Sulfur Radioisotopes</subject><subject>Transducin - physiology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUctKAzEUDaJoffyBwmwUXYzezDPZCFqsFgQXuhRCkrlDIzOTmkyV-vVm2qI7cRVyXvfmhJBjCpcUaHEFQLM4L3l6zuGC0SSn8XSLjCgr0zjNCrZNRj-SPbLv_RuEO6N8l-wmNKF5AiPy-jLDqMIedW9sF8muivRMOql7dOZLrkBbR_fx3NkeTecj00V98OAS_dz2AzmeNbJdVra1nfSRC6qQUPXGHJKdWjYejzbnAXme3L2MH-LHp_vp-OYx1hkvpjHmBQXgSrFMsbTgmNd5CTUHpbhmwDCTqdKp4hI1q5NE5SotkHFkSVVm6QE5W6eGFd8X6HvRGq-xaWSHduEFAyjLjEEQZmuhdtZ7h7WYO9NKtxQUxFCpGPoSQ1-Cg1hVKqbBdrLJX6gWqx_TpsPAn2546bVsaic7bfxvNi9ZiBp0k7Xu0zS4_NdsMbm7TQZiwDms0GGh63UQhk4_DDrhtcFOY2Vc-EdRWfP3i74BSa-mjg</recordid><startdate>19900917</startdate><enddate>19900917</enddate><creator>Korolkov, S.N.</creator><creator>Garnovskaya, M.N.</creator><creator>Basov, A.S.</creator><creator>Chunaev, A.S.</creator><creator>Dumler, I.L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900917</creationdate><title>The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii</title><author>Korolkov, S.N. ; Garnovskaya, M.N. ; Basov, A.S. ; Chunaev, A.S. ; Dumler, I.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496I-e561009bb84b8369e5f570f90bb9c808e4a3bc3b9aec8f22b5b36e89e82d743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antibodies</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Chlamydomonas - physiology</topic><topic>Chlamydomonas reinhardtii</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G-protein</topic><topic>GTP Phosphohydrolases - physiology</topic><topic>GTP-Binding Proteins - isolation & purification</topic><topic>GTP-Binding Proteins - physiology</topic><topic>GTPase</topic><topic>GTPγ[ 35S]</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate) - metabolism</topic><topic>Immunoblotting</topic><topic>Light</topic><topic>Molecular Weight</topic><topic>Proteins</topic><topic>Rhodopsin</topic><topic>Sulfur Radioisotopes</topic><topic>Transducin - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Korolkov, S.N.</creatorcontrib><creatorcontrib>Garnovskaya, M.N.</creatorcontrib><creatorcontrib>Basov, A.S.</creatorcontrib><creatorcontrib>Chunaev, A.S.</creatorcontrib><creatorcontrib>Dumler, I.L.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Korolkov, S.N.</au><au>Garnovskaya, M.N.</au><au>Basov, A.S.</au><au>Chunaev, A.S.</au><au>Dumler, I.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1990-09-17</date><risdate>1990</risdate><volume>270</volume><issue>1</issue><spage>132</spage><epage>134</epage><pages>132-134</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The presence of G-proteins in the eyespot fraction of
Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[
35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent
M
r 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of
Ch. reinhardtii.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2121520</pmid><doi>10.1016/0014-5793(90)81251-I</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Antibodies Binding and carrier proteins Biological and medical sciences Chlamydomonas - physiology Chlamydomonas reinhardtii Fundamental and applied biological sciences. Psychology G-protein GTP Phosphohydrolases - physiology GTP-Binding Proteins - isolation & purification GTP-Binding Proteins - physiology GTPase GTPγ[ 35S] Guanosine 5'-O-(3-Thiotriphosphate) - metabolism Immunoblotting Light Molecular Weight Proteins Rhodopsin Sulfur Radioisotopes Transducin - physiology |
| title | The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii |
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