The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii

The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii

The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[ 35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 2...

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Veröffentlicht in:FEBS letters 1990-09, Vol.270 (1), p.132-134
Hauptverfasser: Korolkov, S.N., Garnovskaya, M.N., Basov, A.S., Chunaev, A.S., Dumler, I.L.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Antibodies
Binding and carrier proteins
Biological and medical sciences
Chlamydomonas - physiology
Chlamydomonas reinhardtii
Fundamental and applied biological sciences. Psychology
G-protein
GTP Phosphohydrolases - physiology
GTP-Binding Proteins - isolation & purification
GTP-Binding Proteins - physiology
GTPase
GTPγ[ 35S]
Guanosine 5'-O-(3-Thiotriphosphate) - metabolism
Immunoblotting
Light
Molecular Weight
Proteins
Rhodopsin
Sulfur Radioisotopes
Transducin - physiology
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Zusammenfassung:The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[ 35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent M r 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of Ch. reinhardtii.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81251-I