Identification and initial characterization of high-affinity [ 3H]dextrorphan binding sites in rat brain
We have identified specific high-affinity [ 3H]dextrorphan binding sites in rat forebrain. [ 3H]Dextrorphan binds saturably and reversibly to an apparently homogeneous class of sites characterized by a B max of 2.62±0.06 pmol/mg protein and K D of 60±4 nM. Glycine and glutamate independently increas...
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Veröffentlicht in: | European journal of pharmacology 1990-07, Vol.189 (1), p.89-93 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We have identified specific high-affinity [
3H]dextrorphan binding sites in rat forebrain. [
3H]Dextrorphan binds saturably and reversibly to an apparently homogeneous class of sites characterized by a B
max of 2.62±0.06 pmol/mg protein and K
D of 60±4 nM. Glycine and glutamate independently increase [
3H]dextrorphan binding in a concentration-dependent manner. The pharmacological profile of [
3H]dextrorphan binding characterized by equilibrium competition experiments, together with these data suggest that [
3H]dextrorphan labels a site at or near the N-methyl-D-aspartate receptor. |
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ISSN: | 0922-4106 0014-2999 1879-0712 |
DOI: | 10.1016/0922-4106(90)90233-N |