Transgenetic studies implicate interactions between homologous PrP isoforms in scrapie prion replication

Transgenic (Tg) mice expressing both Syrian hamster (Ha) and mouse (Mo) prion protein (PrP) genes were used to probe the mechanism of scrapie prion replication. Four Tg lines expressing HaPrP exhibited distinct incubation times ranging from 48 to 277 days, which correlated inversely with HaPrP mRNA...

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Veröffentlicht in:	Cell 1990-11, Vol.63 (4), p.673-686
Hauptverfasser:	Prusiner, Stanley B., Scott, Michael, Foster, Dallas, Pan, Keh-Ming, Groth, Darlene, Mirenda, Carol, Torchia, Marilyn, Yang, Shu-Lian, Serban, Dan, Carlson, George A., Hoppe, Peter C., Westaway, David, DeArmond, Stephen J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Blotting, Northern Blotting, Western Brain - metabolism Brain - microbiology Brain - pathology Cricetinae Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Mesocricetus Mice Mice, Transgenic Molecular and cellular biology Molecular genetics Nerve Degeneration Prions - genetics Prions - physiology PrPSc Proteins Replication RNA, Messenger - genetics Transcription, Genetic Viral Proteins - genetics Virus Replication
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Zusammenfassung:	Transgenic (Tg) mice expressing both Syrian hamster (Ha) and mouse (Mo) prion protein (PrP) genes were used to probe the mechanism of scrapie prion replication. Four Tg lines expressing HaPrP exhibited distinct incubation times ranging from 48 to 277 days, which correlated inversely with HaPrP mRNA and HaPrP C. Bioassays of Tg brain extracts showed that the prion inoculum dictates which prions are synthesized de novo. Tg mice inoculated with Ha prions had ∼10 9 ID 50 units of Ha prions per gram of brain and
ISSN:	0092-8674 1097-4172
DOI:	10.1016/0092-8674(90)90134-Z