Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group

Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group

Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expressi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta biochimica polonica 1998-01, Vol.45 (1), p.27-32
Hauptverfasser: Misiura, K, Wójcik, M, Krakowiak, A, Koziołkiewicz, M, Pawłowska, Z, Pluskota, E, Cierniewski, C S, Stec, W J
Format: Artikel
Sprache:eng
Schlagworte:
Cells, Cultured
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Humans
Lipids - chemistry
Molecular Structure
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - chemistry
Oligonucleotides, Antisense - pharmacology
Plasminogen Activator Inhibitor 1 - pharmacology
Ribonuclease H - metabolism
Ribonucleases - blood
Solubility
Trityl Compounds - chemistry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expression of plasminogen activator inhibitor type-1 within endothelial cells as compared with unconjugated oligonucleotides.
ISSN:0001-527X
1734-154X
DOI:10.18388/abp.1998_4315