Enumeration of Escherichia coli O157 in cattle faeces using most probable number technique and automated immunomagnetic separation

Aims: To determine the numbers of Escherichia coli O157 present in the faeces of naturally infected cattle. Methods and Results: A combination of the most probable number (MPN) technique and automated immunomagnetic separation (AIMS) was used to enumerate E. coli O157 in cattle faeces from both pa...

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Veröffentlicht in:	Letters in applied microbiology 2004, Vol.38 (1), p.56-59
Hauptverfasser:	Fegan, N, Higgs, G, Vanderlinde, P, Desmarchelier, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Feed Animals Biological and medical sciences Cattle Cattle Diseases - microbiology Colony Count, Microbial - methods Edible Grain Escherichia coli Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli O157 - isolation & purification Feces - microbiology Fundamental and applied biological sciences. Psychology grain‐fed cattle Immunomagnetic Separation - methods Microbiology O157 antigen pasture‐fed cattle quantitative data risk assessment
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Zusammenfassung:	Aims: To determine the numbers of Escherichia coli O157 present in the faeces of naturally infected cattle. Methods and Results: A combination of the most probable number (MPN) technique and automated immunomagnetic separation (AIMS) was used to enumerate E. coli O157 in cattle faeces from both pasture‐fed and grain‐fed animals. A total of 22 E. coli O157 positive faecal samples were enumerated for E. coli O157 (10 from pasture‐fed and 12 from grain‐fed animals). The numbers of E. coli O157 in cattle faeces varied from undetectable (
ISSN:	0266-8254 1472-765X 1365-2673
DOI:	10.1046/j.1472-765X.2003.01439.x