A full-length murine 2-5A synthetase cDNA transfected in NIH-3T3 cells impairs EMCV but not VSV replication

Treatment of cells with interferons (IFNs) induces resistance to virus infection. The 2′-5′oligo A (2-5A) synthetase/RNase L is one of the pathways leading to translation inhibition induced by IFN treatment. A murine cDNA encoding the 43-kDa 2-5A synthetase was cloned and sequenced. NIH-3T3 cell clo...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1990-11, Vol.179 (1), p.228-233
Hauptverfasser:	Coccia, Eliana M., Romeo, Giovanna, Nissim, Ahuva, Marziali, Giovanna, Albertini, Roberto, Affabris, Elisabetta, Battistini, Angela, Fiorucci, Gianna, Orsatti, Roberto, Rossi, Giovanni B., Chebath, Judith
Format:	Artikel
Sprache:	eng
Schlagworte:	2',5'-Oligoadenylate Synthetase - genetics 2',5'-Oligoadenylate Synthetase - metabolism Animals Base Sequence Biological and medical sciences Cell Line Cloning, Molecular Encephalomyocarditis virus - genetics Encephalomyocarditis virus - physiology Fundamental and applied biological sciences. Psychology Gene Library Microbiology Molecular Sequence Data Protein Biosynthesis Rabbits Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Restriction Mapping RNA, Messenger - genetics Transcription, Genetic Transfection Vesicular stomatitis Indiana virus - genetics Vesicular stomatitis Indiana virus - physiology Virology Virus Replication
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Zusammenfassung:	Treatment of cells with interferons (IFNs) induces resistance to virus infection. The 2′-5′oligo A (2-5A) synthetase/RNase L is one of the pathways leading to translation inhibition induced by IFN treatment. A murine cDNA encoding the 43-kDa 2-5A synthetase was cloned and sequenced. NIH-3T3 cell clones transfected with this cDNA expressed the enzymatic activity to various extents and exhibited resistance to encephalomyocarditis virus (EMCV) but not to vesicular stomatitis virus replication. The specific resistance to EMCV can be attributed to 2-5A synthetase.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(90)90292-Y