Properties of cattle bone powder-coated composite particles as high-performance and open column liquid chromatographic column packings

Cattle bone powder (CBP) from natural resources was employed as a protein adsorbent instead of chemically synthesized hydroxyapatite (HA). Though a small amount of impurities was detected, CBP possessed a crystallinity similar to HA. Using CBP/40PE prepared from CBP and polyethylene beads (40 μm) by...

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Veröffentlicht in:	Journal of Chromatography A 1998-07, Vol.813 (1), p.21-33
Hauptverfasser:	Honda, Futaba, Honda, Hirotaka, Koishi, Masumi, Matsuno, Takashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Bone and Bones - chemistry Cattle Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Durapatite Fundamental and applied biological sciences. Psychology gamma-Globulins General aspects, investigation methods Hydrogen-Ion Concentration Hydroxyapatite Indicators and Reagents Microscopy, Electron, Scanning Polyethylenes Powders Proteins Spectrophotometry, Ultraviolet X-Ray Diffraction
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Zusammenfassung:	Cattle bone powder (CBP) from natural resources was employed as a protein adsorbent instead of chemically synthesized hydroxyapatite (HA). Though a small amount of impurities was detected, CBP possessed a crystallinity similar to HA. Using CBP/40PE prepared from CBP and polyethylene beads (40 μm) by dry impact blending as an HPLC column packing, considerable correlation was observed between the elution concentrations of proteins and their p I. Such behavior was caused by the relatively large adsorption capacity for basic proteins. CBP/40PE could completely separate γ-globulin from BSA also as an open column chromatographic support, under relatively low concentration.
ISSN:	0021-9673
DOI:	10.1016/S0021-9673(98)00336-7