Intracellular calcium suppresses mechano-electrical transduction current in chick cochlear hair cells

Intracellular calcium concentration ([Ca2+]i) was elevated by photolysis of the caged calcium compound, Nitr-5, and by use of the intrapipette perfusion technique in hair cells dissociated from the chick cochlea. An increase in [Ca2+]i induced an outward-going current at a membrane potential of -40...

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Veröffentlicht in:O.R.L. Journal for oto-rhino-laryngology and its related specialties 1998-09, Vol.60 (5), p.263-266
Hauptverfasser: KIMITSUKI, T, TAIRA, T, KOMUNE, S, KOMIYAMA, S
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Sprache:eng
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Zusammenfassung:Intracellular calcium concentration ([Ca2+]i) was elevated by photolysis of the caged calcium compound, Nitr-5, and by use of the intrapipette perfusion technique in hair cells dissociated from the chick cochlea. An increase in [Ca2+]i induced an outward-going current at a membrane potential of -40 mV, as recorded with an intracellular medium of 160 mM KCl. This current was carried by the Ca2+-activated K+ current. In contrast, an increase of [Ca2+]i induced an inward-going current at -50 mV with a 160 mM CsCl-based intracellular medium. This inward-going current was carried by the Ca2+-activated non-selective cation current. The amplitude of the mechano-electrical transduction current was suppressed by the increase of [Ca2+]i, achieved both by photolysis and by use of the intrapipette perfusion method.
ISSN:0301-1569
1423-0275
DOI:10.1159/000027607