Intracellular calcium suppresses mechano-electrical transduction current in chick cochlear hair cells
Intracellular calcium concentration ([Ca2+]i) was elevated by photolysis of the caged calcium compound, Nitr-5, and by use of the intrapipette perfusion technique in hair cells dissociated from the chick cochlea. An increase in [Ca2+]i induced an outward-going current at a membrane potential of -40...
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Veröffentlicht in: | O.R.L. Journal for oto-rhino-laryngology and its related specialties 1998-09, Vol.60 (5), p.263-266 |
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Sprache: | eng |
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Zusammenfassung: | Intracellular calcium concentration ([Ca2+]i) was elevated by photolysis of the caged calcium compound, Nitr-5, and by use of the intrapipette perfusion technique in hair cells dissociated from the chick cochlea. An increase in [Ca2+]i induced an outward-going current at a membrane potential of -40 mV, as recorded with an intracellular medium of 160 mM KCl. This current was carried by the Ca2+-activated K+ current. In contrast, an increase of [Ca2+]i induced an inward-going current at -50 mV with a 160 mM CsCl-based intracellular medium. This inward-going current was carried by the Ca2+-activated non-selective cation current. The amplitude of the mechano-electrical transduction current was suppressed by the increase of [Ca2+]i, achieved both by photolysis and by use of the intrapipette perfusion method. |
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ISSN: | 0301-1569 1423-0275 |
DOI: | 10.1159/000027607 |