Triplet pregnancy after intracytoplasmic sperm injection of cryopreserved oocytes: case report

Objective: To report a triplet pregnancy that occurred after intracytoplasmic injection of sperm into cryopreserved oocytes. Design: Case report. Setting: Instituto de Ginecologı́a y Fertilidad (IFER), Buenos Aires, Argentina. Patient: A 36-year-old infertile patient with premature ovarian failure and a previous term pregnancy with fresh donated oocytes. Intervention(s): We administered leuprolide acetate for pituitary down-regulation followed by E 2 valerianate in incremental doses until an endometrial lining of >8 mm was observed by ultrasound. Thawing of frozen donated oocytes, intracytoplasmic sperm injection (ICSI), and translaparoscopic fallopian tube ET also were performed. Natural micronized progesterone was administered intravaginally (600 mg/d) before ET. Main Outcome Measure(s): Ultrasound at the 8th week of gestation revealed a triplet pregnancy with active fetal heartbeats. Result(s): A triple intrauterine gestation was achieved with the use of microinjection into cryopreserved oocytes. Conclusion(s): This case illustrates the feasibility of oocyte cryopreservation for clinical use in the era of ICSI.