Statistical design of ELISA protocols

Statistical design of ELISA protocols

This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by usin...

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Veröffentlicht in:Journal of immunological methods 1990-09, Vol.132 (2), p.247-254
Hauptverfasser: BUNCH, D. S, ROCKE, D. M, HARRISON, R. O
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay - methods
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Molecular immunology
Statistics as Topic
Techniques
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Zusammenfassung:This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(90)90036-U