Urine 8-Oxo-7, 8-Dihydro-2′-Deoxyguanosine vs. 5-(Hydroxymethyl) Uracil as DNA Oxidation Marker in Adriamycin-Treated Patients

We measured the base 5-(hydroxymethyl) uracil (HMUra) and the nucleoside 8-oxo-7, 8-dehydro-2′-deoxyguanosine (8-oxo-dGuo) in urine of adriamycin-treated cancer patients. Adriamycin has been shown to generate oxygen free radicals by various mechanisms. HMUra and 8-oxo-dGuo are two known lesions of DNA, produced by oxygen free reaction on thymine and 2′-deoxyguanosine, respectively. HMUra was measured by GC-MS/isotopic dilution and 8-oxo-dGuo by HPLC/EC, both after prepurification by semipreparative HPLC. Here we report the results of a study involving 20 cancer patients treated with flash doses of ADR. We found that urine HMUra is significantly increased (HMUra (nmol/24h): 80.8 ± 8.44 vs. 98.7 ± 6.87; p