The high resolution G- and R-banding pattern in chromosomes of river buffalo (Bubalus bubalis L.)

High resolution G‐ and R‐banding patterns in chromosomes of river buffalo (Bubalus bubalis L.) were obtained by using early (G‐bands) and late (R‐bands) BrdU‐incorporation in synchronized cell cultures. To better characterize the river buffalo chromosomes, GTG‐, GBG‐, and RBG‐techniques were used. The total number of bands achieved were 490 (207 G‐positive, 207 R‐positive, 45 variable, and 31 centromeric regions). Only one common G‐ and R‐banding nomenclature was reported. The number, position and intensity of G bands were highly similar by the structural GTC and the replicating GBG‐techniques. However, the replicating G‐ and R‐bands appeared to be more distinct and reproducible than the structural G‐bands. Some changes in chromosome nomenclature (chromosomes lp, 2p, 5p, and 21) were made when referred to the cattle homologues.