Stain analysis using oligonucleotide probes specific for simple repetitive DNA sequences

Stain analysis using oligonucleotide probes specific for simple repetitive DNA sequences

The use of oligonucleotide fingerprinting is evaluated in practical forensic work, using both artificially and systematically produced stains as well as actual case work material. The probes (CAC) 5 (GTG) 5 are superior because of their individualizing potential in comparatively fresh specimens with...

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Veröffentlicht in:Forensic science international 1990, Vol.47 (1), p.59-70
Hauptverfasser: Roewer, L., Nürnberg, P., Fuhrmann, E., Rose, M., Prokop, O., Epplen, J.T.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Blood Stains
DNA - analysis
DNA - blood
DNA - urine
DNA degradation
DNA Fingerprinting
Female
Homicide
Humans
Male
Medical sciences
Muscles
Oligonucleotide Probes
Public health. Hygiene
Public health. Hygiene-occupational medicine
Repetitive Sequences, Nucleic Acid
Semen
Simple repetitive DNA
Stain analysis
Vagina
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Zusammenfassung:The use of oligonucleotide fingerprinting is evaluated in practical forensic work, using both artificially and systematically produced stains as well as actual case work material. The probes (CAC) 5 (GTG) 5 are superior because of their individualizing potential in comparatively fresh specimens with little DNA degradation, whereas (GACA) 4 still produces substantial information when high molecular weight DNA is lacking. The overall limitations and the advantages of this technology are discussed in detail and compared to the classical minisatellite probes.
ISSN:0379-0738
1872-6283
DOI:10.1016/0379-0738(90)90285-7