Cloning and characterization of a new swine MHC (SLA) class II DQB allele

Major histocompatibility complex (MHC) of pigs is known as swine leukocyte antigen (SLA). The cDNA encoding a new allele of SLA-DQB class II DQ beta-chain was successfully isolated from a CSK miniature pig (derived from Goettingen strain) and characterized by sequence analyses. SLA-DQB cDNA fragment...

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Veröffentlicht in:Journal of Veterinary Medical Science 1998, Vol.60(6), pp.725-729
Hauptverfasser: Hosokawa, T. (Tokyo Univ. (Japan)), Tanioka, Y, Tanigawa, M, Matsumoto, Y, Onodera, T
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Sprache:eng
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Zusammenfassung:Major histocompatibility complex (MHC) of pigs is known as swine leukocyte antigen (SLA). The cDNA encoding a new allele of SLA-DQB class II DQ beta-chain was successfully isolated from a CSK miniature pig (derived from Goettingen strain) and characterized by sequence analyses. SLA-DQB cDNA fragment encoding beta2-domain was amplified by reverse transcriptase-polymerase chain reaction using the sequences preserved in a various vertebrates as primers. Using non-radioisotope technique with the PCR product as a probe, cDNA clone G01 was isolated from a spleen cDNA library, and nucleotide sequence of this clone was determined. This clone encompassed a whole SLA-DQ beta-chain coding region, containing a total length of 161 nucleotides with an open reading frame (ORF) of 786 nucleotides, 5' untranslated region of 15 nucleotides, and 3' untranslated region of 360 nucleotides ending with a canonical polyadenylation signal, followed by a poly A tail. Sequence comparisons of the ORF of this clone with those of known SLA-DQB genes confirmed that this clone is a new allele (SLA-DQB G01). Phylogenetic analysis of the nucleotide sequences of swine, human, and murine MHC class II genes indicated that SLA-DQB was more similar to HLA-DQB1 than H-2A(beta). Comparison of the nucleotide and deduced amino acid sequences among SLA-DQB alleles showed that SLA-DQ beta-chain polymorphism was found almost in beta(1)-domain which contains the antigenic peptide binding sites
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.60.725