Molecular Cloning of Rat Pax4: Identification of Four Isoforms in Rat Insulinoma Cells

Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-P...

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Veröffentlicht in:	Biochemical and biophysical research communications 1998-07, Vol.248 (1), p.153-156
Hauptverfasser:	Tokuyama, Yoshiharu, Yagui, Kazuo, Sakurai, Ken-ichi, Hashimoto, Naotake, Saito, Yasushi, Kanatsuka, Azuma
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Blotting, Northern Cloning, Molecular Homeodomain Proteins - chemistry Homeodomain Proteins - genetics Insulinoma - genetics Islets of Langerhans - chemistry Molecular Sequence Data Paired Box Transcription Factors Pancreatic Neoplasms - genetics Polymerase Chain Reaction Rats RNA, Messenger - analysis RNA, Messenger - genetics Transcription Factors - chemistry Transcription Factors - genetics Tumor Cells, Cultured
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Zusammenfassung:	Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are expressed in RIN cells. These Pax4 variants may regulate the transcriptional activity of Pax4 during the development of pancreatic islets.
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1998.8925