Cloning of the V-ATPase B subunit cDNA from Culex quinquefasciatus and expression of the B and C subunits in mosquitoes

The V-ATPase B subunit cDNA isolated from the midgut and Malpighian tubules of Culex quinquefasciatus larvae has a 1476 bp ORF encoding a 492 amino acids protein with a predicted mass of 54.8 kDa. Northern blot analysis reveals the presence of 1.8 and 4.2 kb transcripts in larvae and a 3.0 kb transc...

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Veröffentlicht in:Insect molecular biology 1998-08, Vol.7 (3), p.223-232
Hauptverfasser: Filippova, M, Ross, L.S, Gill, S.S
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Sprache:eng
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Zusammenfassung:The V-ATPase B subunit cDNA isolated from the midgut and Malpighian tubules of Culex quinquefasciatus larvae has a 1476 bp ORF encoding a 492 amino acids protein with a predicted mass of 54.8 kDa. Northern blot analysis reveals the presence of 1.8 and 4.2 kb transcripts in larvae and a 3.0 kb transcript in pupae. A single 57 kDa protein band is detected in immunoblot analysis of protein extracts from C. quinquefasciatus and A. aegypti larvae. Using antibodies to the B subunits we demonstrate high-level expression of these subunits in ion-transporting cells of caeca and anterior midgut, and in Malpighian tubules and rectum. High V-ATPase expression was also observed in the larval salivary glands, central nervous system neurophile, the thoracic endocrine complex and in imaginal disc.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.1998.00069.x