Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization

Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization

A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross- polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of 15N labeled human ubiquitin. Inter- and intra-residue NOEs are c...

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Veröffentlicht in:Journal of biomolecular NMR 1999-01, Vol.13 (1), p.61-65
Hauptverfasser: Chiarparin, E, Pelupessy, P, Cutting, B, Eykyn, T R, Bodenhausen, G
Format: Artikel
Sprache:eng
Schlagworte:
Carbon 13
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Zusammenfassung:A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross- polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of 15N labeled human ubiquitin. Inter- and intra-residue NOEs are clearly observed in a very time-effective manner. The signal intensities can be easily normalized.
ISSN:0925-2738
1573-5001
DOI:10.1023/A:1008307012647