Kinetics of the transient-phase and steady-state of the monocyclic enzyme cascades

We present a kinetic analysis of the whole course of the reaction, that is, of both the transient-phase and steady-state, of monocyclic enzyme cascade systems. The equations for the rapid equilibrium conditions are obtained as a particular case of the general transient-phase equations. An analysis of the kinetic data allows the determination of the equilibrium and the rate constants if adequate experimental results are available. Finally, our results for the steady-state are compared with those obtained by other authors.