A New Method for Protein Crystallization Using High Pressure

Crystallization methods for proteins have been the subject of decades of development yet protein crystallization remains the limiting step in structural studies. We present here a new method for protein crystallization—based on the use of high pressure—that enabled us to accelerate dramatically the...

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Veröffentlicht in:	Bio/Technology 1990-06, Vol.8 (6), p.547-549
Hauptverfasser:	Visuri, K, Kaipainen, E, Kivimäki, J, Niemi, H, Leisola, M, Palosaari, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Aldose-Ketose Isomerases Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Carbohydrate Epimerases - biosynthesis Carbohydrate Epimerases - genetics Crystalline structure Crystallization Fundamental and applied biological sciences. Psychology Life Sciences Molecular biophysics Proteins research-paper Structure in molecular biology X-Ray Diffraction
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Beschreibung
Zusammenfassung:	Crystallization methods for proteins have been the subject of decades of development yet protein crystallization remains the limiting step in structural studies. We present here a new method for protein crystallization—based on the use of high pressure—that enabled us to accelerate dramatically the growth of glucose isomerase crystals. We think this method may have a more general utility.
ISSN:	0733-222X 1087-0156 2331-3684 1546-1696
DOI:	10.1038/nbt0690-547