Amylase measurement with 2-chloro-4-nitrophenyl maltotrioside as substrate

The use of 2-chloro-4-nitrophenyl maltotrioside (CNP-G3) as substrate to measure amylase (EC 3.2.1.1) activity in serum directly without the use of auxiliary enzymes was evaluated at two centres. The method was precise (within-run C.V.

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Bibliographische Detailangaben
Veröffentlicht in:	Clinica chimica acta 1998-04, Vol.272 (2), p.137-147
Hauptverfasser:	Foo, A.Ying, Bais, Renze
Format:	Artikel
Sprache:	eng
Schlagworte:	2-chloro-4-nitrophenyl maltotrioside (CNP-G3) Amylases - blood Biological and medical sciences Digestive system Humans Hydrogen-Ion Concentration Indicators and Reagents Investigative techniques, diagnostic techniques (general aspects) Measurement of amylase activity Medical sciences Method comparison Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reproducibility of Results Sensitivity and Specificity Substrate Specificity Trisaccharides - metabolism
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Beschreibung
Zusammenfassung:	The use of 2-chloro-4-nitrophenyl maltotrioside (CNP-G3) as substrate to measure amylase (EC 3.2.1.1) activity in serum directly without the use of auxiliary enzymes was evaluated at two centres. The method was precise (within-run C.V.
ISSN:	0009-8981 1873-3492
DOI:	10.1016/S0009-8981(98)00009-6