Molecular cloning of guinea-pig aromatic-L-amino acid decarboxylase cDNA

Guinea-pig aromatic- l-amino acid decarboxylase (DOPA decarboxylase, DDC), but not rat DDC, reacts with the antibody against rat histidine decarboxylase (HDC). For determination of the molecular reaction for this cross-reactivity, a cDNA clone of guinea-pig DDC was isolated. Guinea-pig DDC consists...

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Veröffentlicht in:	Biochemical and biophysical research communications 1990-08, Vol.170 (3), p.1229-1235
Hauptverfasser:	Taketoshi, Masato, Horio, Yoshiyuki, Imamura, Ikuo, Tanaka, Tatsuya, Fukui, Hiroyuki, Wada, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Aromatic-L-Amino-Acid Decarboxylases - genetics Base Sequence Cloning, Molecular Cross Reactions DNA - genetics DNA, Recombinant - analysis Dopa Decarboxylase - analysis Dopa Decarboxylase - genetics Dopa Decarboxylase - immunology genes Guinea Pigs Histidine Decarboxylase - analysis Histidine Decarboxylase - immunology Molecular Sequence Data Rats Restriction Mapping
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Zusammenfassung:	Guinea-pig aromatic- l-amino acid decarboxylase (DOPA decarboxylase, DDC), but not rat DDC, reacts with the antibody against rat histidine decarboxylase (HDC). For determination of the molecular reaction for this cross-reactivity, a cDNA clone of guinea-pig DDC was isolated. Guinea-pig DDC consists of 480 amino acids and its molecular weight is 54,148. The sequence identity of guinea-pig DDC with rat DDC is 86%. Guinea-pig DDC has a region showing 100 % sequence identity with rat HDC, but only 67 % sequence identity with rat DDC, suggesting that this region is related with the cross-reactivity of guinea-pig DDC and anti-rat HDC antibody.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(90)90525-R