There are two major types of hepatitis C virus in Japan

The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene sugges...

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Veröffentlicht in:	Biochemical and biophysical research communications 1990-08, Vol.170 (3), p.1021-1025
Hauptverfasser:	Enomoto, Nobuyuki, Takada, Akira, Nakao, Toshifumi, Date, Takayasu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences DNA, Viral - analysis Fundamental and applied biological sciences. Psychology Genetics Hepatitis C - classification Hepatitis C - genetics Hepatitis, Viral, Human - classification Humans Microbiology Molecular Sequence Data Polymerase Chain Reaction Sequence Homology, Nucleic Acid Virology
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Zusammenfassung:	The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene suggested that HCV can be classified into two types (named K1 and K2) in Japan. Slot blot hybridization of the fragments indicated that 13 were HCV-K1 and 6 were HCV-K2, which show 80% and 67% nucleotide sequence homology, respectively, with that of the prototype.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(90)90494-8