A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge NDV

The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fow...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1990-09, Vol.178 (1), p.297-300
Hauptverfasser:	Boursnell, M.E.G., Green, P.F., Samson, A.C.R., Campbell, J.I.A., Deuter, A., Peters, R.W., Millar, N.S., Emmerson, P.T., Binns, M.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	AGGLUTININE AGGLUTININS AGLUTININAS Animals Antibodies, Monoclonal - immunology AVES DE CORRAL AVIPOXVIRUS Chickens - genetics Chickens - immunology Chickens - microbiology Cloning, Molecular Culture Techniques EXPRESION GENICA EXPRESSION DES GENES Fowlpox virus - genetics GENE GENE EXPRESSION GENE TRANSFER GENES Genes, Viral Genetic Vectors GLICOSIDASAS GLYCOSIDASE GLYCOSIDASES HN Protein - biosynthesis HN Protein - genetics IMMUNITE IMMUNITY INMUNIDAD NEWCASTLE DISEASE VIRUS Newcastle disease virus - enzymology Newcastle disease virus - genetics POULTRY Promoter Regions, Genetic RECOMBINACION RECOMBINAISON RECOMBINANT VACCINES RECOMBINATION Recombination, Genetic SIALIDASE STRAINS SYNTHETIC VACCINES TRANSFERENCIA DE GENES TRANSFERT DE GENE VACCIN SYNTHETIQUE VACUNA SINTETICA VECTEUR DE MALADIE VECTORES VECTORS Viral Vaccines VIRUS DE LA ENFERMEDAD DE NEWCASTLE VIRUS MALADIE DE NEWCASTLE VOLAILLE
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Zusammenfassung:	The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fowlpox virus. Expression was demonstrated in tissue culture, a protein of the correct size for fully glycosylated HN protein being recognized by an HN-specific monoclonal antibody on Western blots. When the recombinant fowlpox virus was inoculated into chickens by intravenous or wing-web routes, antibody which recognizes HN from purified NDV virions wasinoculated. Protective immunity to NDV was generated in the chickens; at the highest dose of vaccine 100% of the chickens tested were protected against challenge with a virulent strain of NDV.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(90)90408-J