Anthocyanin production of Glehnia littoralis callus cultures

A stable callus line of G. littoralis produces a novel anthocyanin cyanidin 3-O-(6-O-(6-O-(E)- feruloyl-β- d-glucopyranosyl )- 2-O-β- d-xylopyranosyl -β- d-glucopyranoside ) . A stable callus line that produces anthocyanins was established from callusderived from a petiole of a Glehnia littoralis seedling and subcultured in the dark. The major anthocyanin which made up about 60% of the total anthocyanins was determined as cyanidin 3-O-(6-O-(6-O-(E)- feruloyl-β- d-glucopyranosyl )-2-O-β- d-xylopyranosyl-β- d-glucopyranoside ) by chemical and spectroscopic analyses. Anthocyanin contents in the cells cultured on B5 basal medium containing NAA (1 mg 1 −1), kinetin (0.01 mg 1 −1) and 3% sucrose reached 14% (dry wt basis) and the productivity has been sustained for 5 years.