Localization of Amidating Enzymes (PAM) in Frog (Rana temporaria) Endocrine Pancreas

Carboxy-terminal alpha -amidation is an essential posttranslational process associated with the activation of many neuroendocrine peptides. The amidating precursor peptidylglycine alpha -amidating monooxygenase (PAM) includes two enzymatic domains, known as peptidylglycine alpha -hydroxylating monooxygenase (PHM) and peptidyl- alpha -hydroxyglicine alpha -amidating lyase (PAL), responsible for the two steps that constitute the amidating reaction. The presence of PAM in endocrine pancreas has received little attention, which has been mainly focused in mammals. To our knowledge, studies in non-mammalian vertebrate pancreas have been carried out only in fishes (Lophius americanus).