Effect of Microtubule Disruption on Cell Adhesion and Spreading

Microtubules have been involved in a variety of cellular processes. In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to...

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Veröffentlicht in:Biochemical and biophysical research communications 1998-05, Vol.246 (3), p.690-695
Hauptverfasser: Kadi, Azzeddine, Pichard, Véronique, Lehmann, Maxime, Briand, Claudette, Braguer, Diane, Marvaldi, Jacques, Rognoni, Jean-Baptiste, Luis, José
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container_issue 3
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container_title Biochemical and biophysical research communications
container_volume 246
creator Kadi, Azzeddine
Pichard, Véronique
Lehmann, Maxime
Briand, Claudette
Braguer, Diane
Marvaldi, Jacques
Rognoni, Jean-Baptiste
Luis, José
description Microtubules have been involved in a variety of cellular processes. In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to purified ECM proteins. This enhanced cell adhesion is mediated by integrins, but is not attributable to quantitative changes in the number of integrin receptors at the cell surface, as determined by flow cytometric analysis. In contrast to attachment, spreading of HT29-D4 cells was reduced by nocodazole treatment in a dose-dependent manner. Thus, microtubule depolymerization appears to increase initial attachment of cells to extracellular matrix, while impeding subsequent cell spreading.
doi_str_mv 10.1006/bbrc.1998.8536
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ispartof Biochemical and biophysical research communications, 1998-05, Vol.246 (3), p.690-695
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Adenocarcinoma - metabolism
adhesion
Antineoplastic Agents - pharmacology
Cell Adhesion
Cell Size
collagen
cytoskeleton
Dose-Response Relationship, Drug
Humans
integrin
Integrins - metabolism
Microtubules - drug effects
Microtubules - metabolism
navelbine
Neoplasm Metastasis
nocodazole
Nocodazole - pharmacology
paclitaxel
Paclitaxel - analogs & derivatives
Paclitaxel - pharmacology
spreading
Taxoids
Tumor Cells, Cultured
Vinblastine - analogs & derivatives
Vinblastine - pharmacology
title Effect of Microtubule Disruption on Cell Adhesion and Spreading
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