Effect of Microtubule Disruption on Cell Adhesion and Spreading
Microtubules have been involved in a variety of cellular processes. In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to...
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Veröffentlicht in: | Biochemical and biophysical research communications 1998-05, Vol.246 (3), p.690-695 |
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creator | Kadi, Azzeddine Pichard, Véronique Lehmann, Maxime Briand, Claudette Braguer, Diane Marvaldi, Jacques Rognoni, Jean-Baptiste Luis, José |
description | Microtubules have been involved in a variety of cellular processes. In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to purified ECM proteins. This enhanced cell adhesion is mediated by integrins, but is not attributable to quantitative changes in the number of integrin receptors at the cell surface, as determined by flow cytometric analysis. In contrast to attachment, spreading of HT29-D4 cells was reduced by nocodazole treatment in a dose-dependent manner. Thus, microtubule depolymerization appears to increase initial attachment of cells to extracellular matrix, while impeding subsequent cell spreading. |
doi_str_mv | 10.1006/bbrc.1998.8536 |
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In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to purified ECM proteins. This enhanced cell adhesion is mediated by integrins, but is not attributable to quantitative changes in the number of integrin receptors at the cell surface, as determined by flow cytometric analysis. In contrast to attachment, spreading of HT29-D4 cells was reduced by nocodazole treatment in a dose-dependent manner. 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In this study, we examined the role of the microtubular system in the adhesion and spreading of the adenocarcinoma cell line HT29-D4. Disruption of microtubules by nocodazole or navelbine resulted in an increase in cell adhesion to purified ECM proteins. This enhanced cell adhesion is mediated by integrins, but is not attributable to quantitative changes in the number of integrin receptors at the cell surface, as determined by flow cytometric analysis. In contrast to attachment, spreading of HT29-D4 cells was reduced by nocodazole treatment in a dose-dependent manner. 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subjects | Adenocarcinoma - metabolism adhesion Antineoplastic Agents - pharmacology Cell Adhesion Cell Size collagen cytoskeleton Dose-Response Relationship, Drug Humans integrin Integrins - metabolism Microtubules - drug effects Microtubules - metabolism navelbine Neoplasm Metastasis nocodazole Nocodazole - pharmacology paclitaxel Paclitaxel - analogs & derivatives Paclitaxel - pharmacology spreading Taxoids Tumor Cells, Cultured Vinblastine - analogs & derivatives Vinblastine - pharmacology |
title | Effect of Microtubule Disruption on Cell Adhesion and Spreading |
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