Determination of alanine aminotransferase in human serum in an open-closed flow injection configuration
Open-closed flow injection systems allow the development of methods based on multidetection by using conventional detectors. The methods proposed here for the determination of alanine aminotransferase (ALT) utilize a photometric detector included in the closed circuit, which allows one to perform fi...
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| Veröffentlicht in: | Journal of biotechnology 1990-04, Vol.14 (1), p.43-52 |
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| container_end_page | 52 |
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| container_title | Journal of biotechnology |
| container_volume | 14 |
| creator | Fernández-Romero, J.M. Luque de Castro, M.D. Valcárcel, M. |
| description | Open-closed flow injection systems allow the development of methods based on multidetection by using conventional detectors. The methods proposed here for the determination of alanine aminotransferase (ALT) utilize a photometric detector included in the closed circuit, which allows one to perform fixed-time and reaction-rate measurements whose sensitivity can be increased by using the sum of the analytical signals. The methods, with determination ranges between 1 and 500 U l
−1 of ALT, feature sampling frequencies up to 60 h
−1. Their application to serum samples provided excellent results, with recoveries between 95 and 106% and averaging at 99.9%. |
| doi_str_mv | 10.1016/0168-1656(90)90016-5 |
| format | Article |
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−1 of ALT, feature sampling frequencies up to 60 h
−1. Their application to serum samples provided excellent results, with recoveries between 95 and 106% and averaging at 99.9%.</description><subject>Alanine Transaminase - blood</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Calibration</subject><subject>Enzymatic activity</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Flow injection analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Humans</subject><subject>Methods</subject><subject>Photometry</subject><subject>Serum</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtu1TAQhq2qqD0tfYMiZVMEi4Ad3-INEirlIlViA2tr6oxbV4l9aicg3h7nnCO6YzHyXP755fkIuWT0HaNMva_Rt0xJ9cbQt4bWspVHZMN6zVvRK35MNv8kp-SslEdKqTCSnZATxpUSQm_I_SecMU8hwhxSbJJvYIQYIjZQm2nOEIvHDAWbEJuHZYLYFMzLtJY1T1uMrRtTwaHxY_pd24_odl4uRR_ul7xzfkleeBgLXhzec_Lz882P66_t7fcv364_3rZOdHxundFMeA1dL7Wrh2jnpNNaApVSUN4pAESDXILoOVeD4mgAQDh1J32vND8nr_e-25yeFiyznUJxONajMC3FamN411NahWIvdDmVktHbbQ4T5D-WUbvytSs8u8KzhtodXyvr2quD_3I34fC8tAda51eHORQHo6_8XCjPMtNpyvjq82GvwwrjV8BsiwsYHQ4hV352SOH_H_kLJXyX2w</recordid><startdate>19900401</startdate><enddate>19900401</enddate><creator>Fernández-Romero, J.M.</creator><creator>Luque de Castro, M.D.</creator><creator>Valcárcel, M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900401</creationdate><title>Determination of alanine aminotransferase in human serum in an open-closed flow injection configuration</title><author>Fernández-Romero, J.M. ; Luque de Castro, M.D. ; Valcárcel, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-c9714f7a2857c8737cc5c775a05540326aaee9e35a48336d63e9aaa4c6b5f8673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Alanine Transaminase - blood</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Calibration</topic><topic>Enzymatic activity</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Flow injection analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Humans</topic><topic>Methods</topic><topic>Photometry</topic><topic>Serum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández-Romero, J.M.</creatorcontrib><creatorcontrib>Luque de Castro, M.D.</creatorcontrib><creatorcontrib>Valcárcel, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández-Romero, J.M.</au><au>Luque de Castro, M.D.</au><au>Valcárcel, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of alanine aminotransferase in human serum in an open-closed flow injection configuration</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>14</volume><issue>1</issue><spage>43</spage><epage>52</epage><pages>43-52</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>Open-closed flow injection systems allow the development of methods based on multidetection by using conventional detectors. The methods proposed here for the determination of alanine aminotransferase (ALT) utilize a photometric detector included in the closed circuit, which allows one to perform fixed-time and reaction-rate measurements whose sensitivity can be increased by using the sum of the analytical signals. The methods, with determination ranges between 1 and 500 U l
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| ispartof | Journal of biotechnology, 1990-04, Vol.14 (1), p.43-52 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alanine Transaminase - blood Analytical, structural and metabolic biochemistry Biological and medical sciences Biotechnology Calibration Enzymatic activity Enzymes and enzyme inhibitors Flow injection analysis Fundamental and applied biological sciences. Psychology General aspects, investigation methods Humans Methods Photometry Serum |
| title | Determination of alanine aminotransferase in human serum in an open-closed flow injection configuration |
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