A Family of AMPA-Selective Glutamate Receptors

Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity were isolated from a rat brain cDNA library. In situ hybridization revealed differential expression patterns of the cognate mRNAs throughout the brain. Functional expression of the...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1990-08, Vol.249 (4968), p.556-560
Hauptverfasser:	Keinänen, Kari, Wisden, William, Sommer, Bernd, Werner, Pia, Herb, Anne, Verdoorn, Todd A., Sakmann, Bert, Seeburg, Peter H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agonists alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Amino Acid Sequence AMPA receptors Animals Base Sequence Biological and medical sciences Brain Brain - metabolism Brain - physiology cDNA Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Complementary DNA Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Genetics Glutamate Glutamate receptors Glutamates - metabolism Glutamates - pharmacology glutamic acid Ibotenic Acid - analogs & derivatives Ibotenic Acid - pharmacology Kainic Acid - pharmacology Kidney cells Kinetics Messenger RNA Molecular Sequence Data Multigene Family Neurons Neurotransmitter receptors Oligonucleotide Probes Organ Specificity Oxadiazoles - pharmacology oxazole propionic acid Oxazoles - pharmacology Pharmacology Physiological aspects Propionic acid Purkinje cells Quisqualic Acid Rats Receptors Receptors, Glutamate Receptors, Neurotransmitter - drug effects Receptors, Neurotransmitter - genetics Receptors, Neurotransmitter - physiology RNA, Messenger - analysis RNA, Messenger - genetics Sequence Homology, Nucleic Acid Vertebrates: nervous system and sense organs
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Zusammenfassung:	Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity were isolated from a rat brain cDNA library. In situ hybridization revealed differential expression patterns of the cognate mRNAs throughout the brain. Functional expression of the cDNAs in cultured mammalian cells generated receptors displaying α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-selective binding pharmacology (AMPA = quisqualate>glutamate>kainate) as well as cation channels gated by glutamate, AMPA, and kainate and blocked by 6,7-dinitroquinoxaline-2,3-dione (CNQX).
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.2166337