Effects of titanium-dental restorative alloy galvanic couples on cultured cells

The potential exists for titanium and amalgams to become galvanically coupled in the oral cavity. While low galvanic corrosion rates have been measured in vivo for titanium‐amalgam or mercury‐free alloy couples, concerns exist over released corrosion products and adverse tissue responses. It was hypothesized in this study that coupling titanium to amalgams or gallium alloys increased the release of metallic corrosion products and decreased cellular activity and function. The effects of titanium coupled and uncoupled to a conventional amalgam, palladium‐enriched spherical high copper amalgam, a dispersed type high copper amalgam, and a mercury‐free gallium alloy were evaluated in 24‐h cell culture tests. Viability, proliferation, and collagen synthesis were evaluated by the uptake of neutral red, 3H‐thymidine, and immunoassay of procollagen, respectively, and compared to cells not exposed to any test material. The gallium alloy‐titanium couple resulted in significant decreases in cellular viability, proliferation, and collagen synthesis as compared to the other coupled and uncoupled samples. Few differences in the cellular responses of the other coupled and uncoupled samples were observed. Atomic absorption analyses indicated increased release of metal ions from the amalgam and gallium alloy samples coupled to titanium as compared to their uncoupled condition, although the differences were not always significant. Galvanic corrosion of amalgam‐titanium couples in the long term may become significant, and further research is needed. Coupling the gallium alloy to titanium may result in increased galvanic corrosion and cytotoxic responses. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 43: 184–191, 1998