Molecular size characterization of bacterial capsular polysaccharide vaccines by high performance liquid chromatography

Bacterial capsular polysaccharides are major virulence factors and some are used as vaccinal antigens. Their molecular size is an important physicochemical criterion which correlates with immunogenicity. This article describes a new application of high performance liquid chromatography (HPLC), based...

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Veröffentlicht in:	Biologicals 1990-04, Vol.18 (2), p.117-121
Hauptverfasser:	Bussat, B., Schulz, D., Arminjon, F., Valentin, C., Armand, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Capsules Bacterial Vaccines - isolation & purification Bacteriology Biological and medical sciences Chromatography, Gel Chromatography, High Pressure Liquid Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Haemophilus influenzae Haemophilus influenzae - analysis Haemophilus influenzae - immunology Haemophilus Vaccines Microbiology Molecular Weight Polysaccharides, Bacterial - isolation & purification Streptococcus pneumoniae - analysis Streptococcus pneumoniae - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Beschreibung
Zusammenfassung:	Bacterial capsular polysaccharides are major virulence factors and some are used as vaccinal antigens. Their molecular size is an important physicochemical criterion which correlates with immunogenicity. This article describes a new application of high performance liquid chromatography (HPLC), based on molecular sieving, for such an evaluation. This HPLC method is rapid, accurate, reproducible, requires only very low amounts of product and presents good correlation with conventional gel permeation chromatography.
ISSN:	1045-1056 1095-8320
DOI:	10.1016/1045-1056(90)90021-Q