Insulin-like growth factor binding protein-3. Organization of the human chromosomal gene and demonstration of promoter activity
Insulin-like growth factor binding protein-3 (IGFBP-3) can modulate the mitogenic and metabolic effects of the insulin-like growth factors (IGFs). IGFBP-3 protein levels are developmentally regulated and influenced by a number of hormonal stimuli both in vitro and in vivo. As a first step toward und...
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Veröffentlicht in: | The Journal of biological chemistry 1990-07, Vol.265 (21), p.12642-12649 |
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Sprache: | eng |
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Zusammenfassung: | Insulin-like growth factor binding protein-3 (IGFBP-3) can modulate the mitogenic and metabolic effects of the insulin-like
growth factors (IGFs). IGFBP-3 protein levels are developmentally regulated and influenced by a number of hormonal stimuli
both in vitro and in vivo. As a first step toward understanding how hormonal and developmental factors regulate IGFBP-3 production,
we are characterizing the human IGFBP-3 chromosomal gene and promoter. Southern analysis demonstrates a single copy of the
IGFBP-3 gene in the human genome. This gene spans 8.9 kilobases; the protein-coding region is divided into four exons while
a fifth exon contains the 3'-untranslated region. Primer extension studies locate the IGFBP-3 mRNA cap site 132 base pairs
5' to the ATG translation initiation codon. On the chromosomal gene, this cap site is located 30 base pairs 3' to the start
of a TATA box and 97 base pairs 3' to a consensus GC upstream promoter element, an organization common to many eukaryotic
promoters. When this potential IGFBP-3 promoter region is placed upstream to the chloramphenicol acetyltransferase reporter
gene, it directs high-level production of chloramphenicol acetyltransferase in transfected COS-1 cells. These observations
suggest an uncomplicated organization for the IGFBP-3 chromosomal gene and promoter in the human genome. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/s0021-9258(19)38392-9 |