Oral or parenteral administration of replication-deficient adenoviruses expressing the measles virus haemagglutinin and fusion proteins: protective immune responses in rodents
AR Fooks, D Jeevarajah, J Lee, A Warnes, S Niewiesk, V ter Meulen, JR Stephenson and JC Clegg Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK. anthony.fooks@camr.org.uk The genes encoding the measles virus (MV) haemagglutinin (H) and fusion (F) proteins were placed under the...
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Veröffentlicht in: | Journal of general virology 1998-05, Vol.79 (5), p.1027-1031 |
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Zusammenfassung: | AR Fooks, D Jeevarajah, J Lee, A Warnes, S Niewiesk, V ter Meulen, JR Stephenson and JC Clegg
Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK. anthony.fooks@camr.org.uk
The genes encoding the measles virus (MV) haemagglutinin (H) and fusion (F)
proteins were placed under the control of the human cytomegalovirus
immediate early promoter in a replication-deficient adenovirus vector.
Immunofluorescence and radioimmune precipitation demonstrated the synthesis
of each protein and biological activity was confirmed by the detection of
haemadsorption and fusion activities in infected cells. Oral as well as
parenteral administration of the H-expressing recombinant adenovirus
elicited a significant protective response in mice challenged with MV.
While the F-expressing adenovirus failed to protect mice, cotton rats
immunized with either the H- or F-expressing recombinant showed reduced MV
replication in the lungs. Antibodies elicited in mice following
immunization with either recombinant had no in vitro neutralizing activity,
suggesting a protective mechanism involving a cell-mediated immune
response. This study demonstrates the feasibility of using oral
administration of adenovirus recombinants to induce protective responses to
heterologous proteins. |
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ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-79-5-1027 |