Inhibition of eukaryote serine/threonine-specific protein kinases by piceatannol

Abstract The protein tyrosine kinase (PTK) inhibitor piceatannol is also an inhibitor of the rat liver cyclic AMP-dependent protein kinase (PKA) catalytic subunit (cAK), rat brain Ca 2+ - and phospholipid-dependent protein kinase C (PKC), avian gizzard Ca 2+ -calmodulin-dependent myosin light chain kinae (MLCK), and of wheat embryo Ca 2+ -dependent protein kinase (CDPK) (IC 50 values 3, 8,12, and 19 µM, respectively). However, a number of piceatannol-related compounds with fewer or no phenolic hydroxy substituents are inactive or very poor inhibitors of these serine/threonine protein kinases. Similarly, the PTK inhibitor ellagic acid is a potent inhibitor of cAK and of PKC (IC 50 values 2 and 8 µM, respectively), whereas the non-phenolic perylene is ineffective as a protein kinase inhibitor. Ellagic acid is a competitive inhibitor of both cAK and of PKC but piceatannol inhibits these enzymes in a fashion that is competitive and non-competitive, respectively. Interaction with calmodulin may contribute to the inhibition of MLCK and CDPK by piceatannol.