Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis
Bordetella pertussis is the causative agent of the respiratory disease pertussis or whooping cough. Btr, an oxygen-responsive transcriptional regulator of B. pertussis, is homologous to the FNR protein of E. coli. Using a murine respiratory model, we observed in the present study that Btr is importa...
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| Veröffentlicht in: | Gene 1998-03, Vol.209 (1), p.51-58 |
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| creator | Wood, Gwendolyn E Khele, Nadia Guiso, Nicole Friedman, Richard L |
| description | Bordetella pertussis is the causative agent of the respiratory disease pertussis or whooping cough. Btr, an oxygen-responsive transcriptional regulator of
B. pertussis, is homologous to the FNR protein of
E. coli. Using a murine respiratory model, we observed in the present study that Btr is important in growth and survival of
B. pertussis in vivo. A titration assay was developed that identified genes containing Btr binding sites including
B. pertussis sodB and
btr,
E. coli aspA and a new
B. pertussis gene,
brg1. The
brg1 gene encodes a protein similar to the LysR family of transcriptional regulators, and its expression is activated threefold by Btr under anaerobic growth conditions but unaffected by Btr aerobically. The nucleotide sequence flanking
brg1 encodes proteins with a similarity to various metabolic enzymes. Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between
brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1. |
| doi_str_mv | 10.1016/S0378-1119(98)00031-6 |
| format | Article |
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B. pertussis, is homologous to the FNR protein of
E. coli. Using a murine respiratory model, we observed in the present study that Btr is important in growth and survival of
B. pertussis in vivo. A titration assay was developed that identified genes containing Btr binding sites including
B. pertussis sodB and
btr,
E. coli aspA and a new
B. pertussis gene,
brg1. The
brg1 gene encodes a protein similar to the LysR family of transcriptional regulators, and its expression is activated threefold by Btr under anaerobic growth conditions but unaffected by Btr aerobically. The nucleotide sequence flanking
brg1 encodes proteins with a similarity to various metabolic enzymes. Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between
brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(98)00031-6</identifier><identifier>PMID: 9583950</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Bordetella pertussis - genetics ; Bordetella pertussis - growth & development ; Bordetella pertussis - pathogenicity ; DNA-Binding Proteins ; Escherichia coli - genetics ; FNR homologue ; Gene Expression Regulation, Bacterial ; Genomic Library ; LysR ; Molecular Sequence Data ; Open Reading Frames ; Oxygen ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - biosynthesis ; Restriction Mapping ; Superoxide Dismutase - biosynthesis ; Superoxide Dismutase - genetics ; Transcription Factors ; Virulence</subject><ispartof>Gene, 1998-03, Vol.209 (1), p.51-58</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-1641331df9512bedbca3a15fbbef51160b9ff4a2090469414adc3d5f36d6f6d3</citedby><cites>FETCH-LOGICAL-c360t-1641331df9512bedbca3a15fbbef51160b9ff4a2090469414adc3d5f36d6f6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(98)00031-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9583950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wood, Gwendolyn E</creatorcontrib><creatorcontrib>Khele, Nadia</creatorcontrib><creatorcontrib>Guiso, Nicole</creatorcontrib><creatorcontrib>Friedman, Richard L</creatorcontrib><title>Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis</title><title>Gene</title><addtitle>Gene</addtitle><description>Bordetella pertussis is the causative agent of the respiratory disease pertussis or whooping cough. Btr, an oxygen-responsive transcriptional regulator of
B. pertussis, is homologous to the FNR protein of
E. coli. Using a murine respiratory model, we observed in the present study that Btr is important in growth and survival of
B. pertussis in vivo. A titration assay was developed that identified genes containing Btr binding sites including
B. pertussis sodB and
btr,
E. coli aspA and a new
B. pertussis gene,
brg1. The
brg1 gene encodes a protein similar to the LysR family of transcriptional regulators, and its expression is activated threefold by Btr under anaerobic growth conditions but unaffected by Btr aerobically. The nucleotide sequence flanking
brg1 encodes proteins with a similarity to various metabolic enzymes. Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between
brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Bordetella pertussis - genetics</subject><subject>Bordetella pertussis - growth & development</subject><subject>Bordetella pertussis - pathogenicity</subject><subject>DNA-Binding Proteins</subject><subject>Escherichia coli - genetics</subject><subject>FNR homologue</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genomic Library</subject><subject>LysR</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Oxygen</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Restriction Mapping</subject><subject>Superoxide Dismutase - biosynthesis</subject><subject>Superoxide Dismutase - genetics</subject><subject>Transcription Factors</subject><subject>Virulence</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9uGyEQxlHVKnWSPkIkTlV72BTMgpdT1UZNGilSDskdsTDYVOvFHdhEeaK-ZlnbyjWnYfT95g_zEXLB2SVnXH17YGLVNZxz_UV3XxljgjfqHVnwbqWbmnXvyeIV-UhOc_5TISbl8oScaNkJLdmC_Lv1MJYYorMlppGmQH8WbBDW02ALeLqGETKdchzX1NISCx5Am7N9uaQPYNFtaEhYVUwD7J9lEzOt5Jgdxt3M24Eee1Y5jpUAusb0XDbUjp4-RZwGGB3sF0joocAwWLoDLFPOMZ-TD8EOGT4d4xl5vP71ePW7ubu_ub36cdc4oVhpuGq5ENwHLfmyB987KyyXoe8hSM4V63UIrV0yzVqlW95a74SXQSivgvLijHw-tN1h-jtBLmYbs5tXGSFN2ax0p5RcqQrKA-gw5YwQzA7j1uKL4czM_pi9P2Y-vtGd2ftj5rqL44Cp34J_rToaUvXvBx3qJ58ioMkuzofxEcEV41N8Y8J_mSCkFA</recordid><startdate>19980316</startdate><enddate>19980316</enddate><creator>Wood, Gwendolyn E</creator><creator>Khele, Nadia</creator><creator>Guiso, Nicole</creator><creator>Friedman, Richard L</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980316</creationdate><title>Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis</title><author>Wood, Gwendolyn E ; Khele, Nadia ; Guiso, Nicole ; Friedman, Richard L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-1641331df9512bedbca3a15fbbef51160b9ff4a2090469414adc3d5f36d6f6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Bordetella pertussis - genetics</topic><topic>Bordetella pertussis - growth & development</topic><topic>Bordetella pertussis - pathogenicity</topic><topic>DNA-Binding Proteins</topic><topic>Escherichia coli - genetics</topic><topic>FNR homologue</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genomic Library</topic><topic>LysR</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Oxygen</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Restriction Mapping</topic><topic>Superoxide Dismutase - biosynthesis</topic><topic>Superoxide Dismutase - genetics</topic><topic>Transcription Factors</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wood, Gwendolyn E</creatorcontrib><creatorcontrib>Khele, Nadia</creatorcontrib><creatorcontrib>Guiso, Nicole</creatorcontrib><creatorcontrib>Friedman, Richard L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wood, Gwendolyn E</au><au>Khele, Nadia</au><au>Guiso, Nicole</au><au>Friedman, Richard L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1998-03-16</date><risdate>1998</risdate><volume>209</volume><issue>1</issue><spage>51</spage><epage>58</epage><pages>51-58</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Bordetella pertussis is the causative agent of the respiratory disease pertussis or whooping cough. Btr, an oxygen-responsive transcriptional regulator of
B. pertussis, is homologous to the FNR protein of
E. coli. Using a murine respiratory model, we observed in the present study that Btr is important in growth and survival of
B. pertussis in vivo. A titration assay was developed that identified genes containing Btr binding sites including
B. pertussis sodB and
btr,
E. coli aspA and a new
B. pertussis gene,
brg1. The
brg1 gene encodes a protein similar to the LysR family of transcriptional regulators, and its expression is activated threefold by Btr under anaerobic growth conditions but unaffected by Btr aerobically. The nucleotide sequence flanking
brg1 encodes proteins with a similarity to various metabolic enzymes. Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between
brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9583950</pmid><doi>10.1016/S0378-1119(98)00031-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | Gene, 1998-03, Vol.209 (1), p.51-58 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Bordetella pertussis - genetics Bordetella pertussis - growth & development Bordetella pertussis - pathogenicity DNA-Binding Proteins Escherichia coli - genetics FNR homologue Gene Expression Regulation, Bacterial Genomic Library LysR Molecular Sequence Data Open Reading Frames Oxygen Promoter Regions, Genetic Recombinant Fusion Proteins - biosynthesis Restriction Mapping Superoxide Dismutase - biosynthesis Superoxide Dismutase - genetics Transcription Factors Virulence |
| title | Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis |
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