Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis
Bordetella pertussis is the causative agent of the respiratory disease pertussis or whooping cough. Btr, an oxygen-responsive transcriptional regulator of B. pertussis, is homologous to the FNR protein of E. coli. Using a murine respiratory model, we observed in the present study that Btr is importa...
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Veröffentlicht in: | Gene 1998-03, Vol.209 (1), p.51-58 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Bordetella pertussis is the causative agent of the respiratory disease pertussis or whooping cough. Btr, an oxygen-responsive transcriptional regulator of
B. pertussis, is homologous to the FNR protein of
E. coli. Using a murine respiratory model, we observed in the present study that Btr is important in growth and survival of
B. pertussis in vivo. A titration assay was developed that identified genes containing Btr binding sites including
B. pertussis sodB and
btr,
E. coli aspA and a new
B. pertussis gene,
brg1. The
brg1 gene encodes a protein similar to the LysR family of transcriptional regulators, and its expression is activated threefold by Btr under anaerobic growth conditions but unaffected by Btr aerobically. The nucleotide sequence flanking
brg1 encodes proteins with a similarity to various metabolic enzymes. Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between
brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(98)00031-6 |