Alteration of endogenous glutathione peroxidase, manganese superoxide dismutase, and glutathione transferase activity in cells transfected with a copper-zinc superoxide dismutase expression vector: explanation for variations in paraquat resistance
Transfection of a human pSV2 (copper-zinc) superoxide dismutase expression vector into murine fibroblasts resulted in stable clones producing increased amounts of copper-zinc superoxide dismutase. A marked increase in endogenous glutathione peroxidase activity (up to 285%) and a smaller increase in...
Gespeichert in:
Veröffentlicht in: | The Journal of biological chemistry 1990-07, Vol.265 (19), p.10872-10875 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Transfection of a human pSV2 (copper-zinc) superoxide dismutase expression vector into murine fibroblasts resulted in stable
clones producing increased amounts of copper-zinc superoxide dismutase. A marked increase in endogenous glutathione peroxidase
activity (up to 285%) and a smaller increase in glutathione transferase activity (up to 16%) also occurred. Manganese superoxide
dismutase activity was decreased in all clones, whereas catalase and NADPH reductase activities were not affected. Alterations
in glutathione peroxidase and manganese superoxide dismutase activities correlated with increases in copper-zinc superoxide
dismutase activity. Whereas all clones were resistant to paraquat, a direct correlation between copper-zinc superoxide dismutase
activity and resistance to paraquat did not exist. In agreement with previous reports clones expressing the highest copper-zinc
superoxide dismutase activity did not display the highest resistance to paraquat. However, there was a direct correlation
between the increase in glutathione peroxidase activity and paraquat resistance (p less than 0.002). |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)38527-8 |