Olga—oligonucleotide primer design program for the Atari ST

A program to facilitate the design of oligonucleotide primners has been devised. Olga is written in draft ANSI standard ‘C’ and makes use of the implementation of Digital Research GEM (Graphics Environment Manager) on the Atari ST Olga is specifically suited to the polymnerase chain reaction (PCR) allowing si analysis of two primer sequences. The advantage of Olga is that it provides in one program analyses for direct repeats, secondary structures and primer dimerization as well as several useful ‘finishing’ tools for workers engaged in PCR optimization and oligonucleotide syntheses.