Crystallization of blood coagulation factor XIII by an automated procedure

Both recombinant blood coagulation factor XIII a-chain and factor XIII isolated from human placenta have been crystallized using a novel robotic system for the automatic screening of crystallization conditions. The monoclinic and orthorhombic crystals obtained are suitable for X-ray analysis.

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Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1990-06, Vol.265 (1), p.110-112
Hauptverfasser:	Hilgenfeld, Rolf, Liesum, Alexander, Storm, Rüdiger, Metzner, Hubert J., Karges, Hermann E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Blood coagulation factor XIII Crystallization Crystallization robotic Enzyme Precursors - isolation & purification Factor XIII - isolation & purification Female Fundamental and applied biological sciences. Psychology Humans placenta Placenta - enzymology Pregnancy Protein crystallization Transglutaminase X-ray crystallography X-Ray Diffraction
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Beschreibung
Zusammenfassung:	Both recombinant blood coagulation factor XIII a-chain and factor XIII isolated from human placenta have been crystallized using a novel robotic system for the automatic screening of crystallization conditions. The monoclinic and orthorhombic crystals obtained are suitable for X-ray analysis.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(90)80896-Q