Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique
A safe, cost effective and accurate immunodetection technique for the rapid identification of Mycobacterium bovis colonies is described. One hundred and thirteen M. bovis isolates were differentiated from other species of mycobacteria using a M. bovis specific monoclonal antibody, with nitrocellulos...
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| Veröffentlicht in: | Veterinary microbiology 1990-05, Vol.22 (4), p.335-340 |
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| container_title | Veterinary microbiology |
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| creator | Veerman, G.M. Kelman, R. Colley, J. Pike, J.G. |
| description | A safe, cost effective and accurate immunodetection technique for the rapid identification of
Mycobacterium bovis colonies is described. One hundred and thirteen
M. bovis isolates were differentiated from other species of mycobacteria using a
M. bovis specific monoclonal antibody, with nitrocellulose membrane as the solid support. The technique confirms
M. bovis identification in only 4 h compared to 3 weeks for conventional antibiotic sensitivity tests. |
| doi_str_mv | 10.1016/0378-1135(90)90020-V |
| format | Article |
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Mycobacterium bovis colonies is described. One hundred and thirteen
M. bovis isolates were differentiated from other species of mycobacteria using a
M. bovis specific monoclonal antibody, with nitrocellulose membrane as the solid support. The technique confirms
M. bovis identification in only 4 h compared to 3 weeks for conventional antibiotic sensitivity tests.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/0378-1135(90)90020-V</identifier><identifier>PMID: 2114051</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; BOVINAE ; Cost-Benefit Analysis ; Fundamental and applied biological sciences. Psychology ; IDENTIFICACION ; IDENTIFICATION ; Immunoblotting ; IMMUNOLOGICAL TECHNIQUES ; Microbiology ; MYCOBACTERIUM ; Mycobacterium bovis - immunology ; Mycobacterium bovis - isolation & purification ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS ; Time Factors</subject><ispartof>Veterinary microbiology, 1990-05, Vol.22 (4), p.335-340</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-681eada628cdcd04f347db3627db21c9b6c10b7d02d133e8c2a147bbfdc275dd3</citedby><cites>FETCH-LOGICAL-c436t-681eada628cdcd04f347db3627db21c9b6c10b7d02d133e8c2a147bbfdc275dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/037811359090020V$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6896697$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2114051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Veerman, G.M.</creatorcontrib><creatorcontrib>Kelman, R.</creatorcontrib><creatorcontrib>Colley, J.</creatorcontrib><creatorcontrib>Pike, J.G.</creatorcontrib><title>Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>A safe, cost effective and accurate immunodetection technique for the rapid identification of
Mycobacterium bovis colonies is described. One hundred and thirteen
M. bovis isolates were differentiated from other species of mycobacteria using a
M. bovis specific monoclonal antibody, with nitrocellulose membrane as the solid support. The technique confirms
M. bovis identification in only 4 h compared to 3 weeks for conventional antibiotic sensitivity tests.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>BOVINAE</subject><subject>Cost-Benefit Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>Immunoblotting</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>Microbiology</subject><subject>MYCOBACTERIUM</subject><subject>Mycobacterium bovis - immunology</subject><subject>Mycobacterium bovis - isolation & purification</subject><subject>TECHNIQUE IMMUNOLOGIQUE</subject><subject>TECNICAS INMUNOLOGICAS</subject><subject>Time Factors</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtrFDEYhoNY6rb6B0QhFyL2YjSnSSY3gpRqhVVBtLchxzUyM9kmmcL-e2d2l720N_kI7_MdeF8AXmH0HiPMPyAqugZj2r6T6EoiRFBz9wSscCdoQ1pGnoLVCXkGLkr5ixBikqNzcE4wZqjFK7D5qbfRQZvGEPOga8o7GJ0fawzR6hrTCFOA33Y2GW2rz3EaoEkPscCpxHEDNXSpQtOnWpdvHIZpTM5Xb_e9c_0zxvvJPwdnQffFvzjWS_D7882v69tm_ePL1-tP68YyymvDO-y105x01lmHWKBMOEM5mV-CrTTcYmSEQ8RhSn1nicZMGBOcJaJ1jl6Ct4e525zmtaWqIRbr-16PPk1FCdm1mAjxKIhbTiViZAbZAbQ5lZJ9UNscB513CiO1BKEWl9XispJI7YNQd3Pb6-P8yQzenZqOzs_6m6Oui9V9yHq0sZww3knO5XLmywMWdFJ6k2fk-3q_Ay_ix4PoZ0cfos-q2OhH613McwDKpfj_I_8BHcyu0w</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Veerman, G.M.</creator><creator>Kelman, R.</creator><creator>Colley, J.</creator><creator>Pike, J.G.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900501</creationdate><title>Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique</title><author>Veerman, G.M. ; Kelman, R. ; Colley, J. ; Pike, J.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-681eada628cdcd04f347db3627db21c9b6c10b7d02d133e8c2a147bbfdc275dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>BOVINAE</topic><topic>Cost-Benefit Analysis</topic><topic>Fundamental and applied biological sciences. 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Mycobacterium bovis colonies is described. One hundred and thirteen
M. bovis isolates were differentiated from other species of mycobacteria using a
M. bovis specific monoclonal antibody, with nitrocellulose membrane as the solid support. The technique confirms
M. bovis identification in only 4 h compared to 3 weeks for conventional antibiotic sensitivity tests.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2114051</pmid><doi>10.1016/0378-1135(90)90020-V</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 1990-05, Vol.22 (4), p.335-340 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79851277 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences BOVINAE Cost-Benefit Analysis Fundamental and applied biological sciences. Psychology IDENTIFICACION IDENTIFICATION Immunoblotting IMMUNOLOGICAL TECHNIQUES Microbiology MYCOBACTERIUM Mycobacterium bovis - immunology Mycobacterium bovis - isolation & purification TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS Time Factors |
| title | Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique |
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