Rapid confirmatory identification of Mycobacterium bovis using a dot blotting immunodetection technique

A safe, cost effective and accurate immunodetection technique for the rapid identification of Mycobacterium bovis colonies is described. One hundred and thirteen M. bovis isolates were differentiated from other species of mycobacteria using a M. bovis specific monoclonal antibody, with nitrocellulos...

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Veröffentlicht in:	Veterinary microbiology 1990-05, Vol.22 (4), p.335-340
Hauptverfasser:	Veerman, G.M., Kelman, R., Colley, J., Pike, J.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences BOVINAE Cost-Benefit Analysis Fundamental and applied biological sciences. Psychology IDENTIFICACION IDENTIFICATION Immunoblotting IMMUNOLOGICAL TECHNIQUES Microbiology MYCOBACTERIUM Mycobacterium bovis - immunology Mycobacterium bovis - isolation & purification TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS Time Factors
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Zusammenfassung:	A safe, cost effective and accurate immunodetection technique for the rapid identification of Mycobacterium bovis colonies is described. One hundred and thirteen M. bovis isolates were differentiated from other species of mycobacteria using a M. bovis specific monoclonal antibody, with nitrocellulose membrane as the solid support. The technique confirms M. bovis identification in only 4 h compared to 3 weeks for conventional antibiotic sensitivity tests.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/0378-1135(90)90020-V