Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.

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Bibliographische Detailangaben
Veröffentlicht in:Current genetics 1990-05, Vol.17 (5), p.409-411
Hauptverfasser: LEUNG, H, LEHTINEN, U, KARJALAINEN, R, SKINNER, D, TOOLEY, P, LEONG, S, ELLINGBOE, A
Format: Artikel
Sprache:eng
Schlagworte:
Anti-Bacterial Agents - pharmacology
Ascomycota - drug effects
Ascomycota - genetics
Biological and medical sciences
Biotechnology
blast
Blotting, Southern
Cosmids
DNA
Drug Resistance, Microbial - genetics
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
hygromycin B
Hygromycin B - pharmacology
Methods. Procedures. Technologies
Plasmids
transformation
Transformation, Genetic
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Beschreibung
Zusammenfassung:Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00334519