Sequence Analysis of the actA Gene of Listeria monocytogenes Isolated from Human

Sequence Analysis of the actA Gene of Listeria monocytogenes Isolated from Human

The region encoding proline-rich units of actA genes was amplified from 24 strains of Listeria monocytogenes using polymerase chain reaction (PCR). PCR products of 13 strains showed the expected size of 623bp, whereas those of 11 strains showed a short size of 518bp. The shortening of these PCR prod...

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Veröffentlicht in:MICROBIOLOGY and IMMUNOLOGY 1998, Vol.42(2), pp.129-132
Hauptverfasser: Moriishi, Kohji, Terao, Michinori, Koura, Minako, Inoue, Satoshi
Format: Artikel
Sprache:eng
Schlagworte:
actA
Adult
Aged
Bacteriology
Base Sequence
Biological and medical sciences
Child, Preschool
Fundamental and applied biological sciences. Psychology
Genes, Bacterial - genetics
Genetics
Humans
Infant
Infant, Newborn
Listeria monocytogenes
Listeria monocytogenes - genetics
Microbiology
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA - methods
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Zusammenfassung:The region encoding proline-rich units of actA genes was amplified from 24 strains of Listeria monocytogenes using polymerase chain reaction (PCR). PCR products of 13 strains showed the expected size of 623bp, whereas those of 11 strains showed a short size of 518bp. The shortening of these PCR products resulted from the deletion of one proline-rich unit. These results indicate that ActA proteins are divided into at least two different types which are unrelated to bacterial serotypes.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1998.tb02261.x