Adhesion of Monocytes to Vascular Cell Adhesion Molecule-1-Transduced Human Endothelial Cells: Implications for Atherogenesis

Adhesion of Monocytes to Vascular Cell Adhesion Molecule-1-Transduced Human Endothelial Cells: Implications for Atherogenesis

To study the role of vascular cell adhesion molecule-1 (VCAM-1) in monocyte recruitment and atherogenesis, we constructed a recombinant adenovirus, AdRSVrVCAM-1, carrying the rabbit VCAM-1 cDNA. We have previously shown that AdRSVrVCAM-1-transduced human umbilical vein endothelial cells (HUVECs) sup...

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Veröffentlicht in:Circulation research 1998-05, Vol.82 (8), p.871-878
Hauptverfasser: Gerszten, Robert E, Lim, Yaw-Chyn, Ding, Han T, Snapp, Karen, Kansas, Geoffrey, Dichek, David A, Cabanas, Carlos, Sanchez-Madrid, Francisco, Gimbrone, Michael A, Rosenzweig, Anthony, Luscinskas, Francis W
Format: Artikel
Sprache:eng
Schlagworte:
Adenoviruses, Human
Animals
Antigens, CD - analysis
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cell Adhesion - physiology
Cell Line
Cells, Cultured
Endothelium, Vascular - cytology
Endothelium, Vascular - physiology
Gene Transfer Techniques
Genetic Vectors
Humans
Integrin alpha4
Integrin beta1 - analysis
Jurkat Cells
Kidney
L-Selectin - analysis
Medical sciences
Membrane Glycoproteins - analysis
Monocytes - cytology
Monocytes - physiology
P-Selectin - analysis
Rabbits
T-Lymphocytes - immunology
T-Lymphocytes - physiology
Transfection
Umbilical Veins
Vascular Cell Adhesion Molecule-1 - biosynthesis
Vascular Cell Adhesion Molecule-1 - physiology
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Zusammenfassung:To study the role of vascular cell adhesion molecule-1 (VCAM-1) in monocyte recruitment and atherogenesis, we constructed a recombinant adenovirus, AdRSVrVCAM-1, carrying the rabbit VCAM-1 cDNA. We have previously shown that AdRSVrVCAM-1-transduced human umbilical vein endothelial cells (HUVECs) support the adhesion of CD4 CD45RO memory T lymphocytes under laminar flow conditions. We now demonstrate that AdRSVrVCAM-1-transduced HUVECs support the adhesion of peripheral blood monocytes at a shear stress of
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.82.8.871