Yeast Ku as a regulator of chromosomal DNA end structure

During telomere replication in yeast, chromosome ends acquire an S-phase--specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1998-05, Vol.280 (5364), p.741-744
Hauptverfasser: Gravel, S, Larrivee, M, Labrecque, P, Wellinger, R.J
Format: Artikel
Sprache:eng
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Zusammenfassung:During telomere replication in yeast, chromosome ends acquire an S-phase--specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross-linking experiments demonstrated that Ku is bound to telomeric DNA. These results show that Ku plays a direct role in establishing a normal DNA end structure on yeast chromosomes, conceivably by functioning as a terminus-binding factor. Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres
ISSN:0036-8075
1095-9203
DOI:10.1126/science.280.5364.741