Near-field fluorescence microscopy of cells

We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane pro...

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Veröffentlicht in:	Ultramicroscopy 1998-03, Vol.71 (1), p.303-309
Hauptverfasser:	Enderle, Th, Ha, T., Chemla, D.S., Weiss, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacillus subtilis - chemistry Bacterial Proteins - analysis Biological and medical sciences Biological techniques and instrumentation biomedical engineering Biology Cell membranes. Ionic channels. Membrane pores Cell structures and functions Cells Conventional optical microscopes Erythrocyte Membrane - chemistry Erythrocyte Membrane - parasitology Erythrocyte Membrane - ultrastructure Exact sciences and technology Fluorescence Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Green fluorescent protein Green Fluorescent Proteins Indicators and Reagents Instruments, apparatus, components and techniques common to several branches of physics and astronomy Luminescent Proteins Malaria Membrane Proteins - analysis Membranes Microscopic methods, specifically for biological specimens Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Molecular and cellular biology Molecular biophysics Near-field optical microscopy Optical instruments, equipment and techniques Optics and Photonics Physics Plasmodium falciparum Proteins Protozoan Proteins - analysis Red-blood cells Sensitivity and Specificity Spectroscopy : techniques and spectras Spectroscopy in NFOM Spores, Bacterial - chemistry Spores, Bacterial - ultrastructure Tissues, organs and organisms biophysics
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Beschreibung
Zusammenfassung:	We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.
ISSN:	0304-3991 1879-2723
DOI:	10.1016/S0304-3991(97)00075-2